Sanne Hoeks

TH17 within first three months of life 51 4 Fig E3 Journal of Allergy and Clinical Immunology 2014 133891-894.e5DOI: (10.1016/j.jaci.2013.09.022) FIGURE E3. Proliferation and cytokine production after activation with anti-CD3. PBMCs were activated by plate-bound anti-CD3. A, Proliferation was measured after 4 days of culture by 3H-labeled thymidine incorporation for 16 hours. B and C, After 6 days, culture supernatants were harvested and analyzed for IFNg and IL-13 production, respectively. D, ratio between IFNg and IL-13 in the culture supernatant. *P < .05. Antigen-presenting cells (APCs) in CB have an important role in the induction of Treg cells from naive T cells. Alloreactions between naive T cells and APCs show higher numbers of Treg cells when CB-derived APCs are used. 2 We wondered whether this capacity of CB APCs is also observed in infant-derived APCs. We first tested the number of induced Treg cells when fluorescence activated cell sorting–sorted CD4 + CD45RA + CD45RO - naive T cells were activated with autologous APCs (Fig 2, D ). In contrast to CB samples, all other samples had Treg-cell numbers comparable to adult samples. Next, we activated naive T cells from samples from donors aged 3 to 12 months with autologous APCs from CB and vice versa. Because of low cell numbers, we had to pool results from all infant samples. CB APCs induced more FOXP3 + T cells than did APCs derived from older donors (Fig 2, E ). Nevertheless, CB T cells