Sanne Hoeks

TH17 within first three months of life 53 4 Fig 2 f Allergy and Clinical Immunology 2014 133891-894.e5DOI: (10.1016/j.jaci.2013.09.022) FIGURE 2. T H cells have a high propensity to become FOXP3 + Treg cells in the first 12 months of life, but T H 17 cells can already be induced at 3 months. PBMCs were activated by plate-bound anti-CD3. A, IL-17–producing T H cells were assessed after 6 days of culture and 4 hours of restimulation with phorbol 12-myristate 13-acetate and ionomycin in the presence of monensin. B, The concentration of IL-17 in the supernatants of PBMCs cultured for 6 days. C, FOXP3 expression was assessed by using flow cytometry with intracellular staining after 6 days of PBMC culture. D, FOXP3 expression after 6 days of culture of CD4 + CD25-CD45RA + CD45RO - T cells with CD3- APCs. E, FOXP3 expression in CD4 T cells cultured with APCs from the same donor but isolated at a different donor age. A and B, CB n= 5, 3 months n= 4, 6 months n= 3, 12 months n= 3, adult n= 6; C, CB n= 5, 3 months n= 4, 6 months n= 3, 12 months n= 3, adult n= 6; D, CB n= 5, 3 months n= 2, 6 months n= 2, 12 months n= 3, adult n= 6; E, CB n= 2, 3 months n= 3, 6 months n= 3, 12 months n= 3. *P <.05, **P <.01, compared with adult. Data as mean ± SEM.