Daan Pieren

115 Co-expression of TIGIT and Helios by CD8+ T cells SUPPLEMENTARY FIGURES 0 20 40 60 80 100 % CD69 + cells of subset (Relative to control) ns ns *** *** * *** ns *** * 0 1 3 0 1 3 0 1 3 0 1 3 Time (days) TIGIT Helios + + + - - + - - 0 20 40 60 80 100 % CD25 + cells of subset (Relative to control) 0 1 3 0 1 3 0 1 3 0 1 3 Time (days) TIGIT Helios + + + - - + - - ns * * *** p = 0.07 ** *** p = 0.07 ** A B Supplementary Figure 1. Kinetics of activation (CD69 and CD25) in TIGIT/Helios cell subsets. Total PBMCs were cultured in the presence of anti-CD3 for one or three days. Frequencies of ( A ) CD69 + cells and ( B ) CD25 + cells within the indicated TIGIT/Helios cell subsets (n=13) were determined before (day zero) and after stimulation (day one and three). For day one and three after stimulation, frequencies are depicted as relative to their unstimulated control sample. Statistical significance of data presented in the line graphs was determined using Friedman test (with Dunn’s post-test). (*p <0.05, **p <0.01, ***p <0.001, ns=not significant). 4