Daan Pieren

149 Regulatory KIR+RA+ CD8+ T cells accumulate with age A B PCA1 (25.5%) PCA2 (13.4%) C KIR (PE) NKG2A (APC) CD27 (BV510) CD8 (FITC) T NAIVE NKG2A + RA + T EMRA KIR + RA + CD45RA + CD8 + T cells CD45RA + KIR - NKG2A - CD8 + T cells -200 -100 0 100 200 -100 -50 0 50 100 Donor #1 -200 -100 0 100 200 -100 -50 0 50 100 Donor #2 -200 -100 0 100 200 -100 -50 0 50 100 Donor #3 -200 -100 0 100 200 -100 -50 0 50 100 Donor #4 -200 -100 0 100 200 -100 -50 0 50 100 Donor #5 -200 -100 0 100 200 -100 -50 0 50 100 Donor #6 KIR + RA + T NAIVE T EMRA NKG2A + RA + Other donor Supplementary Figure 2. Sorted T-cell subsets and donor variation observed in unsupervised principal component analysis (PCA). ( A,B ) Flow cytometry plots indicate the four FACS-sorted subsets, first gated on CD56 - CD4 - CD19 - CD3 + CD8 + T cells: T NAIVE cells (CD8 + CD45RA + KIR - NKG2A - CD27 + ), T EMRA cells (CD8 + CD45RA + KIR - NKG2A - CD27 - ), KIR + RA + T cells (CD8 + CD45RA + KIR + NKG2A - ), and NKG2A + RA + T cells (CD8 + CD45RA + KIR - NKG2A + ). ( C ) Unsupervised principal component analysis (PCA) performed on the total transcriptome (34,745 genes) within each of the sorted cell subsets is shown for each of the six donors. 5