Daan Pieren

150 Chapter 5 4.32 0 z-score T EMRA KIR + RA + NKG2A + RA + Compared toT NAIVE Compared toT NAIVE T EMRA KIR + RA + NKG2A + RA + Molecular and Cellular function Pathway analysis Supplementary Figure 3. Ingenuity Pathway Analysis (IPA) performed on T EMRA , KIR + RA + and NKG2A + RA + T cells compared to T NAIVE cells to identify molecular and cellular function pathways. Ingenuity Pathway Analysis (IPA) was performed by comparing the differentially expressed genes (DEGs) of T EMRA , KIR + RA + , and NKG2A + RA + T-cell subsets to T NAIVE cells to identify molecular and cellular functions of these cell subsets. Pathways identified in this analysis were then compared between T EMRA , KIR + RA + , and NKG2A + RA + T-cell subsets by aligning their hierarchical clustered heat maps to identify in which shared and unique pathways these subsets differed from each other and from T NAIVE cells. Colors in the heat map indicate the z-score calculated for each identified pathway, with cutoff values: -Log( p -value) >1.5, z-score with an absolute value > 2.0. Statistical significance ( p <0.05) was calculated by Fisher’s Exact Test.