Daan Pieren

151 Regulatory KIR+RA+ CD8+ T cells accumulate with age B A 0 20 40 60 80 100 0 20 40 60 80 % T N in total CD8 + 0 20 40 60 80 100 0 20 40 60 80 % T CM in total CD8 + 0 20 40 60 80 100 0 10 20 30 40 50 %T EM in total CD8 + 0 20 40 60 80 100 0 20 40 60 % T EMRA in total CD8 + r = -0.898 p <0.001 r = 0.274 ns r = 0.543 p <0.001 r = 0.518 p <0.001 T N cells T CM cells T EM cells T EMRA cells r = 0.214 ns NKG2A + RA + cells % TIGIT + cells in CD8 + T cells % TIGIT + cells in CD8 + T cells T NAIVE T CM T EM T EMRA KIR + RA + NKG2A + RA + 0 2000 4000 6000 8000 10000 12000 TIGIT expression in subset (MFI) *** *** *** *** * 0 20 40 60 80 100 0 2 4 6 8 10 % NKG2A + RA + in total CD8 + Supplementary Figure 4. Proportion of TIGIT + cells within CD8 + T-cell subsets. ( A ) Relationship between the proportion of T NAIVE , T CM , T EM , T EMRA , or NKG2A + RA + cells within the CD8 + T-cell population and the proportion of TIGIT + cells within the CD8 + T-cell population. ( B ) Expression (Median Fluorescent Intensity, MFI) of TIGIT within the indicated CD8 + T-cell subsets in healthy individuals (20-82 years of age, n=50). Correlations ( r values) were assessed by Spearman test. Statistical significance of data presented in the bar graph (means ± s.d.) was determined using Friedman test (with Dunn’s post-test). (* p <0.05, *** p <0.001). 5