Daan Pieren

158 Chapter 5 Supplementary Table 4. Antibodies used in this study Fluorochrome Clone Isotype Manufacturer Catalogue nr. Dilution Whole blood Assay Dilution Surface markers CD3 BV421 UCHT1 Mouse IgG1, kappa Biolegend 300434 1:200 - CD3 FITC UCHT1 Mouse IgG1, kappa Biolegend 300440 1:200 1:100 CD4 PE-Cy7 RPA-T4 Mouse IgG1, kappa Biolegend 300512 1:100 - CD4 PerCP-Cy5.5 RPA-T4 Mouse IgG1, kappa Biolegend 300530 1:200 1:100 CD8a BB515 RPA-T8 Mouse IgG1, kappa BD Horizon 564526 1:100 - CD8a BUV395 RPA-T8 Mouse IgG1, kappa BD Horizon 563795 1:100 1:200 CD8a FITC RPA-T8 Mouse IgG1, kappa Biolegend 301050 1:50 - CD19 BV786 HIB19 Mouse IgG1, kappa Biolegend 302240 1:100 - CD27 BV510 O323 Mouse IgG1, kappa Biolegend 302836 1:50 1:100 CD45 Pacific Blue HI30 Mouse IgG1, kappa Biolegend 304029 - 1:400 CD45RA BV605 HI100 Mouse IgG2b, kappa Biolegend 304134 1:800 1:400 CD45RA PerCP-Cy5.5 HI100 Mouse IgG2b, kappa Biolegend 304122 1:50 - CD56 PE-Cy7 HCD56 Mouse IgG1, kappa Biolegend 318318 1:100 - CD69 BV785 FN50 Mouse IgG1, kappa Biolegend 310932 1:100 1:100 CD122 PE-Cy7 TU27 Mouse IgG1, kappa Biolegend 339014 1:50 - CD226 BV786 DX11 Mouse IgG1, kappa BD 742497 1:100 - KIR2D PE NKVFS1 Mouse IgG1, kappa Miltenyi Biotec 130-092-688 1:400 1:400 KIR3DL1 PE 5.133 Mouse IgG1 Miltenyi Biotec 130-095-205 1:25 1:200 NKG2A APC REA110 Recombinant human IgG1 Miltenyi Biotec 130-113-563 1:400 1:800 TIGIT PE-eFluor610 MBSA43 Mouse IgG1, kappa eBioscience 61-9500-42 1:50 -