Daan Pieren

159 Regulatory KIR+RA+ CD8+ T cells accumulate with age Supplementary Table 4. Continued. Fluorochrome Clone Isotype Manufacturer Catalogue nr. Dilution Whole blood Assay Dilution Fixable Viability Stain FVS780 N/A N/A BD Horizon 565388 1:2000 - Intracellular markers CD3 FITC UCHT1 Mouse IgG1, kappa Biolegend 300440 1:200 - CD107a PE-Cy7 H4A3 Mouse IgG1, kappa Biolegend 328618 1:50 - Helios PE-Cy7 22F6 Armenian hamster IgG Biolegend 137236 1:200 - IFN-gamma BUV737 4S.B3 Mouse IgG1, kappa BD 564620 1:50 - IL-2 BV785 MQ1-17H12 Rat IgG2a, kappa Biolegend 500348 1:50 - TNF-alpha PerCP-Cy5.5 Mab11 Mouse IgG1, kappa Biolegend 502926 1:50 - 5