Daan Pieren

56 Chapter 2 aCD3 aCD3 + IL-2 aCD3 aCD3 + IL-2 0 2 4 6 8 10 12 aCD3 aCD3 + IL-2 aCD3 aCD3 + IL-2 0 2 4 6 8 10 12 *** aCD3 aCD3 + IL-2 aCD3 aCD3 + IL-2 0 2 4 6 8 10 12 14 16 18 *** Young (2 months) Aged (28 months) % of max CellTrace Th cells Tc cells Treg cells Proliferation (Day 4) Proliferation (Fold change Cellterace MFI) Young Aged Young Aged Young Aged A B aCD3 - aCD3 + IL-2 aCD3 - aCD3 + IL-2 aCD3 - aCD3 + IL-2 aCD3 - aCD3 + IL-2 aCD3 - aCD3 + IL-2 aCD3 - aCD3 + IL-2 Supplementary Figure 3. T cells of aged mice do not proliferate in response to exogenous IL-2. ( A ) Histograms depict the proliferative response of Th, Tc, and Treg cells of young (2 months old) and aged (28 months old) mice measured after four days of exposure to a low anti-CD3 stimulatory strength or low anti-CD3 + exogenous IL-2. Histograms show one mouse that is representative of its age group. ( B ) Graphs show proliferation by fold change in CellTrace MFI of Th, Tc, and Treg cells of young (n=6, 2 months old) and aged (n=6, 28 months old) mice in response to anti-CD3 +/- exogenous IL-2. Mean ± SD; *** p < 0.001 for difference between young and aged mice using Two-way ANOVA.