Daan Pieren

60 Chapter 2 Th PD-1 + PD-1 - Young Aged Day 0 Day 2 PD-1 + PD-1 - Day 0 Day 2 CD69 CD25 Tc 95.7 2.3 1.8 0.2 99.6 0.2 0.2 0.0 1.6 16.2 0.3 81.9 8.9 28.4 1.7 61.0 1.0 6.2 0.2 92.6 96.3 1.0 1.9 0.7 99.6 0.1 0.2 0.0 9.5 14.9 1.2 74.4 37.2 21.5 1.5 39.8 99.3 0.3 0.3 0.1 94.5 5.0 0.3 0.2 47.3 15.5 6.3 30.9 27.6 23.0 4.5 44.9 97.0 1.0 1.9 0.1 99.0 0.9 0.1 0.0 32.9 23.0 1.9 42.2 Supplementary Figure 8 Supplementary Figure 8. Gating of PD-1 + or PD-1 - Th and Tc cells expressing CD25 + and CD69 + before and after stimulation. Plots show the expression of CD25 and CD69 in PD-1 + and PD-1 - Th and Tc cells before and after two day exposure to an intermediate stimulatory strength. Plots depict analyses of one young (2 months old) and one aged (28 months old) mouse that represent their age group.