Daan Pieren

88 Chapter 3 SUPPLEMENTARY FIGURES CD4 (APC) CD8 (APC-H7) 59.1 37.6 2.66 Ercc1 -/ ∆ 7 0 1 2 3 4 5 % CD4 - CD8 - of CD3 + cells A B Supplementary Figure 1. Gating and proportion of CD3 + CD4 - CD8 - cells in Ercc1 -/ Δ 7 mice. Representative FACS plot ( A ) shows expression of CD8 and CD4 within the live CD3 + T cells of Ercc1 -/ Δ 7 mice. Numbers indicate the percentage of events in each gate as part of all events in the FACS plot. Bar graph ( B ) shows the frequency of CD4 - CD8 - cells within the live CD3 + T-cell subset that was assessed in the spleen of Ercc1 -/ Δ 7 mice (n=10) by flow cytometry. Bar graph shows the mean ± SD.