Daan Pieren

90 Chapter 3 A B WT Young (2 months) WT Aged (22 months) 0 20 40 60 % Treg of naive CD4 + *** 0 20 40 60 % Treg of memory CD4 + ns Supplementary Figure 3. FoxP3 + Tregs accumulate within the naive CD4 + T cell subset of wild-type aged mice. The frequencies of ( A ) naive Tregs (FoxP3 + of CD44 Lo CD4 + CD3 + cells) and ( B ) memory Tregs (FoxP3 + of CD44 Hi CD4 + CD3 + cells) were determined in the spleens of WT young (n=6, 2 months old) and aged mice (n=6, 22 months old). Bar graphs show mean ± SD; ** p < 0.01, *** p < 0.001, ns = not statistically significant for the difference between groups using unpaired Student’s t test, two-tailed.