Daan Pieren

92 Chapter 3 0 2 4 6 8 10 % Naive Tc reg *** 0 5 10 15 % Memory Tc reg * Ercc1 +/+ A B 9.5 1.6 CD122 (PE-CF594) PD-1 (BV786) Naive Tc reg Memory Tc reg C Ercc1 -/ ∆ 7 Supplementary Figure 5. Tc reg cells accumulate within the memory Tc-cell subset of Ercc1 - deficient mice. Representative FACS plots ( A ) show the gating of naive and memory Tc reg cells based on the expression of CD122 and PD-1. The frequencies of ( B ) Treg cells among naive Tc cells (PD-1 + CD122 + of CD44 Lo CD4 - CD3 + cells) and ( C ) Treg cells among memory Tc cells (PD-1 + CD122 + of CD44 Hi CD4 - CD3 + cells) were determined in the spleen of Ercc1 +/+ (n=6, 4 months of age) and Ercc1 -/ Δ 7 (n=7, 4 months of age) mice. Bar graphs show mean ± SD; * p < 0.05, *** p < 0.001 for the difference between groups using unpaired Student’s t test, two-tailed.