Daan Pieren

93 Compromised DNA repair promotes the accumulation of regulatory T cells A B Ercc1 +/+ Ercc1 -/ ∆ 7 Ercc1 -/ ∆ 7 + Rapamycin 70 80 90 100 % Th of naive CD4 + ** ns ns 0 20 40 60 80 100 % Th of memory CD4 + ns *** *** 0 10 20 30 % Treg of naive CD4 + *** 0.06 ns 0 20 40 60 80 % Treg of memory CD4 + *** *** ns 0 20 40 60 % naive Tc of CD3 + * ns * 0 2 4 6 8 10 % memory Tc of CD3 + * ns ns 0 2 4 6 8 10 % CD25 + of Th ** *** * 0 2 4 6 8 10 % CD122 + of Th ns ** * 0 1 2 3 % PD-1 + of Th ns ns 0.05 0 10 20 30 % CD25 + of Th ns *** *** 0 20 40 60 80 100 % CD122 + of Th ** ns ** 0 10 20 30 40 % PD-1 + of Th ns ns 0.09 70 80 90 100 % CD25 + of Treg ** 0.06 ns 0 2 4 6 8 10 % CD122 + of Treg ns ** * 0 10 20 30 40 % PD-1 + of Treg ** ns * 40 60 80 100 % CD25 + of Treg *** *** ns 0 2 4 6 8 10 % CD122 + of Treg ns ns * 0 20 40 60 80 100 % PD-1 + of Treg *** *** ns 0 2 4 6 8 10 % CD25 + of Tc ** ns 0.07 0 10 20 30 % CD25 + of Tc ns *** *** 0 5 10 15 % CD122 + of Tc ns ns ns 0 20 40 60 80 100 % CD122 + of Tc *** ns *** 0 2 4 6 8 10 % PD-1 + of Tc *** * * 0 10 20 30 % PD-1 + of Tc * ns ns Naive Th Memory Th Naive Treg Memory Treg Naive Tc Memory Tc T-cell subsets %CD25 + %CD122 + %PD-1 + Supplementary Figure 6. The effect of eRapa on CD25 + , CD122 + , and PD-1 + cell frequencies within naive and memory T-cell subsets. Bar graphs ( A ) indicate the frequencies of the indicated naive and memory T-cell subsets. Within each of these T-cell subsets the frequencies of CD25 + , CD122 + , and PD-1 + cells were determined ( B ) in the spleen of Ercc1 +/+ (n=6, blue bars), Ercc1 -/ Δ 7 (n=7, orange bars), and Ercc1 -/ Δ 7 treated with eRapa (n=6, grey bars). Bar graphs show mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, ns = not statistically significant for the difference between groups using one-way ANOVA corrected with Holm-Sidak test for multiple comparisons. 3