Daan Pieren

94 Chapter 3 0 20 40 60 80 100 % Proliferated Tc aCD3 aCD28 IL-2 - - - + - - + + - + - + * ns ns ns ns ** 0 20000 40000 60000 80000 100000 120000 CD25 expression Tc (MFI) aCD3 aCD28 IL-2 - - - + - - + + - + - + * ns ns ns 0 20000 40000 60000 80000 100000 120000 CD25 expression Th (MFI) aCD3 aCD28 IL-2 - - - + - - + + - + - + * **** ns ns 0 20 40 60 80 100 % Proliferated Tc *** *** ns ns aCD3 aCD28 IL-2 - - - + - - + + - + - + 0 20 40 60 80 100 % Proliferated Th *** *** ns ns aCD3 aCD28 IL-2 - - - + - - + + - + - + A B C D E F G H 0 20 40 60 80 100 % Proliferated Th aCD3 aCD28 IL-2 - - - + - - + + - + - + * ns 0.10 ns ns ns 0 10000 20000 30000 40000 CD25 expression Th (MFI) aCD3 aCD28 IL-2 - - - + - - + + - + - + ns 0.11 ns ns ns ns 0 10000 20000 30000 40000 CD25 expression Th (MFI) aCD3 aCD28 IL-2 - - - + - - + + - + - + ns ** * ns ns ns Ercc1 +/+ Ercc1 -/ ∆ 7 Ercc1 -/ ∆ 7 + Rapamycin Ercc1 +/+ Ercc1 -/ ∆ 7 Ercc1 -/ ∆ 7 + Rapamycin WT Young (2 months) WT Aged (22 months) WT Young (2 months) WT Aged (22 months) Supplementary Figure 7. Compromised DNA repair limits T-cell receptor/Interleukin-2 me- diated Th- and Tc-cell responsiveness.