Mylène Jansen

SF biomarker changes upon KJD 347 16 Supplementary Table 4 : The median change in concentration of each analyte over the 6 week distraction period for clinical responders and non-responders Analyte Change in responders Change in non-responders Effect size P- value Activin A (ng/ml) -6940 -7443 -503.2 0.64 LTBP2 (ng/ml) 270.0 207.4 -62.7 0.84 TGF β -1 (ng/ml) 4128 918 -3210 0.036 FGF-2 (pg/ml) 297.1 1.23 -295.9 0.014 TIMP-1 (ng/ml) 194.5 508.7 314.2 0.37 TSG-6 (ng/ml) 2.5 65.0 62.5 0.24 IL-6 (pg/ml) 33.87 241.7 207.9 0.84 MCP-1 (pg/ml) 170.1 50.78 -119.3 0.54 IL-8 (pg/ml) 7.105 -0.19 -7.3 0.99 MMP3 (ng/ml) 102.3 315.8 213.5 0.99 Medians are shown. The calculated effect size is the difference between the medians. Figure 4B also includes the 95% confidence intervals. Between group comparisons were by Mann-Whitney U test. FGF-2: basic fibroblast growth factor; IL-6: interleukin 6; IL-8: interleukin 8; LTBP2: latent-transforming growth factor beta-binding protein 2; MCP-1: monocyte chemoattractant protein 1; MMP3, matrix metalloproteinase-3; TGF β -1: transforming growth factor beta 1; TIMP-1: tissue inhibitor of metalloproteinases 1; TSG-6: TNF stimulated protein-6. Activin A LTBP2 TGF β -1 FGF-2 TIMP-1 TSG-6 IL-6 MCP-1 IL-8 Activin A LTBP2 0.25 TGF β -1 -0.35* 0.35* FGF-2 <0.01 -0.02 0.27 TIMP-1 0.07 0.15 0.07 0.05 TSG-6 0.41* 0.20 0.01 0.01 0.35* IL-6 0.16 0.23 0.01 -0.15 0.24 0.79*** MCP-1 0.35* -0.45* -0.45* 0.35* -0.06 0.20 0.07 IL-8 0.15 0.49* -0.02 -0.42* -0.25 0.47* 0.55** -0.16 MMP3 <0.01 0.45* 0.04 -0.03 0.69** -0.10 0.11 -0.32* -0.07 Supplementary Figure 1 : Correlation between change of analytes in the synovial fluid of participants over 3 weeks of knee joint distraction. Spearman rank tests were performed to determine correlations between the change in levels of synovial fluid analytes over 3 weeks of distraction period (concentration at 3 weeks-baseline concentrations). Correlation coefficients were calculated using all available participant data and the mean of 2 repeated (duplicate) measures for each synovial fluid sample. Strength of correlation by Spearman R coefficient is shown: * (Light gray shading): Low positive (negative) correlation, 0.30 to 0.49 (−0.30 to −0.49); ** (Mid gray shading): Moderate positive (negative) correlation, 0.50 to 0.69 (−0.50 to −0.69); *** (Dark gray shading): High positive (negative) correlation, 0.70 to 0.89 (−0.70 to −0.89). FGF-2: basic fibroblast growth factor; IL- 6: interleukin 6; IL-8: interleukin 8; LTBP2: latent-transforming growth factor beta-binding protein 2; MCP-1: monocyte chemoattractant protein 1; MMP3, matrix metalloproteinase-3; TGF β -1: transforming growth factor beta 1; TIMP-1: tissue inhibitor of metalloproteinases 1; TSG-6: TNF stimulated protein-6.