Franny Jongbloed

106 CHAPTER 4 Figure S3. Schematic overview of the experimental design. After an acclimation period for all groups of 7 days on SDS chow, the long-term experiments started with diets given for either 10- or 14 days. At day -3 relative to induction of ischemia-reperfusion injury (IRI) the short-term dietary intervention and gene expression profiling experiments were started. At day 0, animals used for transcriptomic profiling were sacrificed for further analyses. In the other groups, blood collection was followed by induction of bilateral renal IRI for 37 minutes and collection of blood one day later. Mice were followed until day 7 after IRI, after which they were sacrificed.