Franny Jongbloed

113 4 A PROTEIN-FREE DIET PROTECTS AGAINST RENAL IRI Table S3. Top 10 overrepresented canonical pathways after the meta-analysis including fasting, dietary restriction and macronutrient free diets ranked by their –log P- value Meta-analysis II (including 3-day 30% DR) Canonical Pathway Pathway Category P- value Genes Ratio Z-score LPS/IL-1 Mediated Inhibition of RXR Function Nuclear Receptor Signaling 4.68 E -05 10/221 (4.5%) N/A NRF2-mediated Oxidative Stress Response Cellular Stress and Injury 8.92 E -05 9/193 (4.7%) N/A PXR/RXR Activation Nuclear Receptor Signaling 3.59 E -04 5/65 (7.7%) N/A Noradrenaline and Adrenaline Degradation Degradation/Utilization/ Assimilation; Hormones 5.25 E -04 4.40 (10.0%) N/A Aryl Hydrocarbon Receptor Signaling Cell Cycle Regulation; Apoptosis; Xenobiotic Metabolism, Nuclear Receptor Signaling 2.08 E -03 6/140 (4.3%) N/A Superpathway of Cholesterol Biosynthesis Fatty Acids and Lipids Biosynthesis, Sterol Biosynthesis 2.23 E -03 3/28 (10.7%) N/A Glutathione-mediated Detoxification Degradation/Utilization/ Assimilation; Detoxification 2.47 E -03 3/29 (10.3%) N/A Circadian Rhythm Signaling Neurotransmitters and Other Nervous System Signaling 3.59 E -03 3/33 (9.1%) N/A Retinoate Biosynthesis I Vitamin (A) Biosynthesis; Cofactors, Prosthetic Groups and Electron Carriers Biosynthesis 3.91 E -03 3/34 (8.8%) N/A Dopamine Degradation Degradation/Utilization/ Assimilation; Amines and Polyamines Degradation 4.25 E -03 3/35 (8.6%) N/A The top 10 overrepresented pathways derived from the 279 DEPS in common between 3-days of fasting, 2 weeks 30% DR, 3 days of a protein-free and 3 days of a 30% DR diet. These pathways are mostly involved in regulation of nuclear receptor signalling (5 out of 10), biosynthesis signalling (2 out of 10) and cellular stress and injury (2 out of 10).