Franny Jongbloed

115 4 A PROTEIN-FREE DIET PROTECTS AGAINST RENAL IRI Table S5. Overview of the dietary interventions, the groups and numbers of mice used for phenotypical and transcriptional endpoints Dietary Intervention Model Duration (days) Number of mice Follow- up (days) Parameters measured (A) Overview IRI long-term experiments Control SDS C57BL/6 14 10 7 Survival, body weight, kidney function Control C57BL/6 14 12 7 Survival, body weight, kidney function Fat-free C57BL/6 14 6 7 Survival, body weight, kidney function Pair-fed fat- free C57BL/6 14 6 7 Survival, body weight, kidney function Carbohydrate-free C57BL/6 14 6 1 Survival, body weight, kidney function Pair-fed carbohydrate-free C57BL/6 14 6 7 Survival, body weight, kidney function Protein-free C57BL/6 10 6 7 Survival, body weight, kidney function (B) Overview IRI short-term experiments Control C57BL/6 3 4 7 Survival, body weight, kidney function Protein-free C57BL/6 3 4 7 Survival, body weight, kidney function AL Control (control to 3-day 30% DR) C57BL/6 3 4 7 Survival, body weight, kidney function 3 days 30% DR C57BL/6 3 6 7 Survival, body weight, kidney function (C) Overview of gene transcription experiments Control SDS (control 2-week 30% DR) C57BL/6 14 4 0 Gene expression profiling in kidney tissue 2-week 30% DR C57BL/6 14 5 0 Gene expression profiling in kidney tissue Control SDS (control 3-day fasting) C57BL/6 3 5 Gene expression profiling in kidney tissue 3-day fasting C57BL/6 3 4 0 Gene expression profiling in kidney tissue Control C57BL/6 3 5 0 Gene expression profiling in kidney tissue Protein-free C57BL/6 3 5 0 Gene expression profiling in kidney tissue Fat-free C57BL/6 3 4 0 Gene expression profiling in kidney tissue Carbohydrate-free C57BL/6 3 5 0 Gene expression profiling in kidney tissue