Franny Jongbloed

43 2 FASTING PROTECTS AGED-OVERWEIGHT MICE SUPPLEMENTARY DATA Table S1. Top genes upregulated in aged mice fasted for three days Genes AGED upregulated Symbol Log FR P- value Cytochrome P450, family 4, subfamily a, polypeptide 14 Cyp4a14 5.842 5.46 E -06 3-hydroxy-3-methylglutaryl-CoA synthase 2 HMGCS2 5.125 1.48 E -06 Phosphoenolpyruvate carboxykinase 1 PCK1 3.260 1.83 E -04 Acyl-CoA thioesterase 1 ACOT1 2.609 6.18 E -06 Pyruvate dehydrogenase kinase, isozyme 4 PDK4 2.508 2.44 E -03 Solute carrier family 38, member 3 SLC38A3 2.365 5.57 E -05 Cytochrome P450, family 4, subfamily a, polypeptide 11 Cyp4a11 2.250 2.15 E -05 Mitochondrial amidoxime reducing component 1 MARC1 2.209 6.23 E -03 WD repeat and SOCS box containing 1 WSB1 2.020 3.64 E -06 Inhibitor of DNA binding 1 ID1 1.937 6.18 E -06 Kv channel-interacting protein 2 Kcnip2 1.913 6.50 E -06 inhibitor of DNA binding 3 ID3 1.903 7.35 E -06 acyl-CoA thioesterase 1 Acot1 1.902 4.94 E -06 betaine--homocysteine S-methyltransferase BHMT 1.890 2.60 E -02 insulin-like growth factor binding protein 1 IGFBP1 1.860 8.55 E -03 group-specific component (vitamin D binding protein) GC 1.853 3.60 E -03 apolipoprotein D APOD 1.808 3.82 E -03 fibrinogen alpha chain FGA 1.802 9.00 E -04 microsomal glutathione S-transferase 1 MGST1 1.792 6.30 E -06 nuclear factor, erythroid 2-like 2 NFE2L2 1.767 4.56 E -03 fibrinogen gamma chain FGG 1.766 1.29 E -02 complement component 3 C3 1.756 1.18 E -04 aldehyde dehydrogenase family 1, subfamily A7 Aldh1a7 1.722 2.02 E -06 glutathione S-transferase alpha 5 GSTA5 1.708 1.63 E -05 amylase, alpha 1A (salivary) AMY1A 1.706 1.16 E -03 acyl-CoA thioesterase 2 ACOT2 1.705 1.48 E -06 ATP-binding cassette, sub-family B (MDR/TAP), member 1B Abcb1b 1.662 5.84 E -06 aldehyde dehydrogenase 1 family, member A1 ALDH1A1 1.655 4.12 E -05 arginase 2 ARG2 1.641 8.57 E -05 cytochrome c oxidase subunit VIb polypeptide 2 (testis) COX6B2 1.624 1.98 E -06 Top gene lists of upregulated genes in aged-overweight mice fasted for three days, with corresponding symbols, log fold ratios and P- values. All genes with a fold change >5 (log fold ratio (-)1.609) are listed.