Franny Jongbloed

75 3 PROTECTION OF FASTING ON IRINOTECAN TOXICITY SUPPLEMENTARY DATA Arrival Balb/c mice acclimatization Day -22 Subcutaneous C26 injection Day -15 Start fasting/ ad libitum Day -3 Irinotecan or vehicle injection ip 8 am Day 0 Sacrifice groups 3 to 6 8 pm Day 0 RNA isolation Day 0 Figure S1. Schematic overview of the experimental set-up, including a timeline from arrival of the mice until sacrifice and subsequent RNA isolation for further analyses. Ip = intraperitoneal.