Franny Jongbloed

83 4 A PROTEIN-FREE DIET PROTECTS AGAINST RENAL IRI Figure 1. A protein-free diet protects against renal IRI. (A) Three days of 30% DR did not protect against renal IRI. (B) Serum urea levels before and at day one after renal IRI did not differ between mice fed ad libitum (AL) for three days of 30% DR and a control diet. (C) Three days of protein-free diet improved survival compared with AL fed controls. (D) Serum urea levels of AL fed protein- free mice were significantly better compared to AL fed control mice (P<0.05). (E) Three days of protein-free diet resulted in 30% calorie restriction. (F) Mice fed a protein-free diet or a 30% DR diet for three days lost significantly more body weight than mice fed the control diet. Body weight changes did not differ between the protein-free diet and 3-day 30% DR. AL Control= ad libitum fed SDS chow, Control= control diet for the macronutrient diets, protein free= ad libitum fed protein- free diet. *= significance with P< 0.05.