Marilen Benner

CHAPTER 4 102 SUPPLEMENTAL INFORMATION CD56 CD3 2nd Term 1st 1st MMC PB 57.8% 1.8% 81.4% 1.7% 15.4% 75.3% 4.4% 17.4% 51.7% 7% 39.2% T NKT NK 9.4% 0.9% 80.3% NKT cells PB MMC 1st 2nd Term 0 5 10 15 20 %CD3+CD56+ of CD45+ *** ** Term 1st 1st 1st NK bright cells NK dim cells 98.1% 1.9% CD16+ CD16- CD56 CD16 CD3-CD56+ PB MMC 1st 2nd Term 0 20 40 60 80 100 %CD16- of CD56+ PB MMC 1st 2nd Term 0 25 50 75 100 %CD16+ of CD56+ D E C ^^^ ^^^ ^^^ ** *** ^^^ ^^^ ^^^ ** *** F I H PB MMC 1st 2nd Term 0 5 10 15 %CCR4-CCR6-CXCR3+ of CD4+ p<0.1 * T helper 1 T helper 2 T helper 17 CD8+T cells PB MMC 1st 2nd Term 0 20 40 60 80 100 %CD8+ of CD3+ ^ * * * ^ CD4+T cells CD4+ PB MMC 1st 2nd Term 0 20 40 60 80 100 %CD4+ of CD3+ ^^^ ^^ *** *** * CD3 CD8 CD4 81.4% 1.7% 15.4% PB MMC 1st 2nd Term 0 2 4 6 8 10 20 40 60 80 100 %CCR4+CCR6-CXCR3+ of CD4+ ^^^ ^^^ ^^ ^^ PB MMC 1st 2nd Term 0 2 4 6 8 10 20 40 60 80 100 %CCR4+CCR6+CXCR3- of CD4+ ^^ * PB 62.5% 16.5% 16.5% 1.84% 37.5% SS CCR6 CCR6+ CD4+ CCR6- CCR4 CXCR3 8% 3.6% CD4 Lymphocytes CD25 Helios FOXP3 CD8 CD127 CD4+ CD25HiCD127low/neg CD25Hi FOXP3+Helios+ G ^^ ^^^ ** *** Naive CD4 T cells PB MMC 1st 2nd Term 0 20 40 60 80 100 %CD45RA+CCR7+ of CD4+ CCR7 CD45RA 5.28% 52.8% Naive EM ^^^ ^ *** CD4 Effector memory PB MMC 1st 2nd Term 0 20 40 60 80 100 %CD45RA-CCR7- of CD4+ A B FS FS TOF SS FS SS CD45 CD14 CD19 Granulocytes excl excl Monocytes/ macrophages CD45+ CD45+ excl granul. CD45+ excl granul./mono. Lymphocytes Singlets Singlets Supplemental Figure S1. Gating strategies and frequencies of menstrual blood and decidual tissue derived lymphocyte frequencies assessed by manual analysis. Related to Figure 1.