Marilen Benner

CHAPTER 4 104 B C D FS FS TOF SS CD45 FS SS A ● ●●●●● ● ●●●●● ●●●●●●●● ●●●●●●●● ●● ●●●●●●●●●●● ●● ●● ● ● ●●●●●● ●● ●●●●●● ●● ●●● ●● ●●●●●●●●●● ● ● ●●●●● ● ●●●● ●●●●●●●●●● Number of model features Regularization Threshold Model Cross Validation Error Rate 6.80 6.05 5.29 4.54 3.78 3.02 2.27 1.51 0.76 0.00 0 25 50 75 100 0 1 1 4 4 7 14 19 27 37 57 71 81 85 ● ● ● Cross Validation Error Rate Feature False Discovery Rate cv.min cv.1se CD45+ Singlets Singlets Lymphocytes Cluster C Cluster D Cluster E Cluster F 0 2 4 6 CD16 HLA−DR 0 2 4 6 CD4 0.0 2.5 5.0 0.0 CD25 CD56 0 3 6 2.5 5.0 7.5 CD8 −2 0 2 4 CD3 0 2 4 CD19 −1 0 1 2 3 CD14 −2 0 2 4 6 Cluster B Cluster A Overall marker expression Marker expression within cluster CD14 0.32 0.79 1.38 1.97 2.56 CD4 −0.17 0.86 2.15 3.44 4.73 CD8 0.03 0.74 1.61 2.49 3.36 CD3 0.49 1.19 2.06 2.94 3.81 0.54 1.46 2.60 3.74 4.89 CD16 HLA-DR 0.33 1.11 2.09 3.07 4.05 CD25 0.09 0.93 1.98 3.03 4.07 CD19 −0.09 0.29 0.77 1.24 1.72 Supplemental Figure S2. CITRUS modelling of general decidual lymphocyte assessment. Related to Figure 1.