Marilen Benner

CHAPTER 7 200 Univariate analysis Data were analyzed using R v.4.0.2 and the ggpubr, ggplot2, ggsignif, tidyr packages. Non- parametric Mann-Whitney test was performed. Values of p <0.05 were considered statistically significant. ACKNOWLEDGEMENTS We thank Negisa Seyed Toutounchi for assistance during the sectioning days, and Rindert Hartman and Niklas Bruse for assistance during data formatting. We would also like to thank F. Paganelli and M.R. de Zoete from the Microbiome Center Utrecht for technical assistance for 16S rRNA gene sequencing, and the Utrecht Exposome Hub of Utrecht Life Sciences (www.uu.nl/ exposome), funded by the Executive Board of Utrecht University. M.B. is supported by a junior researcher grant of the Radboud Institute for Molecular Life Sciences.