Marilen Benner

CHAPTER 7 212 Supplementary Table T2. Immune features assessed after antibiotic treatment in mice Technique Tissue Assessed parameters Flow cytometry Spleen T cells^ Placenta T cells MLN T cells ILN T cells Peritoneal cavity T cells Spleen B cells^^ Placenta B cells Peritoneal cavity B cells Placenta NK_CD3 - CD11b+ CD301+ CD49b+ F4/80+ Luminex Splenocytes after 24h stimulation IL-2 IL-6 IL-10 TNFa IL-1b IFNg IL-22 Amniotic fluid IL-2 IL-6 IL-10 TNFa IL-1b IL-4 IL-5 qPCR Colon FOXP3 Gata3 Rory T-Bet Placenta FOXP3 Gata3 Rory T-Bet IL-10 ^T cell panel: CD4pos, activated CD4pos, Th1 cells, Th2 cells, Th1Th2 ratio, Th17 cells, Treg cells, CD4pos, Neuropilin- 1pos percent of Treg cells ^^ B cell panel: CD19+, CD1dHi CD5+, CD5+ CD1d+, CD1dHi CD21Hi, B10, CD5-, CD1dlow, CD5+, CD1dlow, T2MZP, CD24 - CD21+ CD35+, CD24+ CD21- CD35-, CD24+ CD21+ CD35+, CD5 - CD23-, CD5+ CD23-, CD5+ CD23+, CD1d histogram, CD5 histogram, CD21 histogram, CD23 histogram, CD24 histogram