Marilen Benner

UTERINE LYMPHOCYTES GAIN EXPERIENCE DURING PREGNANCY 31 2 Figure 2. CD4+ and CD8+ T cells subsets in peripheral blood (PBMC), menstrual blood (MMC) and term decidua (DPMC). (A) Representative staining for CD45RA and CD45RO on CD3+ T cells and CD45RA and CCR7 on CD4+ and CD8+ T cells from PBMC, MMC, and DPMC. (B) Expression of CD45RA and CD45RO on CD3+ T cells (PBMC n=17, MMC n=16, and DPMC n=12. (C) CD4+ and CD8+ T cells are separated into four subsets based on the expression of CD45RA and CCR7. Naive T cell (CD45RA+CCR7+); effector T cell, Teff (CD45RA+CCR7-); effector memory T cell, EM (CD45RA-CCR7-); central memory T cell, CM (CD45RA-CCR7+) (PBMC n=17, MMC n=11, and DPMC n=8). Lines indicate mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 (non-parametric Kruskal-Wallis with Dunns post-hoc test)