Marilen Benner

UTERINE LYMPHOCYTES GAIN EXPERIENCE DURING PREGNANCY 33 2 Figure 4. Distribution of CD4+ Th cell subsets in peripheral blood (PBMC), menstrual blood (MMC), and term decidua (DPMC) and production of IFN- γ and IL-17 by CD4+ T cells. (A, C) Representative gating for CCR6, CXCR3, and CCR4 on CD4+ T cells and for IFN- γ and IL-17 by CD4+ T cells from PBMC, MMC, and DPMC. (B) Th1, Th2, Th17, and nonconventional Th1 (Th1-like) CD4+ T cells can be classified based on the expression of CCR6, CXCR3, and CCR4 37. CCR6-CXCR3+CCR4-, Th1; CR6+CXCR3+CCR4-, Th1-like; CCR6- CXCR3-CCR4+, Th2; CCR6+CXCR3-CCR4+, Th17 (PBMC n=17, MMC n=11, DPMC n=7). (D) Production of IFN- γ and IL-17 by CD4+ T cells from PBMC (n=12), MMC (n=15), and DPMC (n=7). *p < 0.05, **p < 0.01, and ***p < 0.001 (lines indicate mean, nonparametric Kruskal-Wallis with Dunns post-hoc test).