Marilen Benner

CHAPTER 3 76 Supplementary Figure S2. tSNE analysis of decidual CD4+ T cells . Related to Figure 1. Representative tSNE plots of one decidual CD4+ T cell sample (6-12wk), highlighting CD25, FOXP3, HELIOS, PD1, CD45RA and TIGIT. Three separate CD4+ T cell populations are identified i) CD25 HI FOXP3+HELIOS+, ii) PD1 HI and iii) CD45RA+. TIGIT is co-expressed by CD25 HI and PD1 HI cells but not by CD45RA+ cells. TIGIT is also expressed by a large proportion of the remaining CD4+ T cells, these TIGIT+ cells are clearly identified in Fig 1B bottom panels. tSNE X P 20 E 200 | 1000 T 0.5 tSNE Y P 20 E 200 | 1000 T 0.5 FOXP3 HELIOS CD25 TIGIT CD25 HI FOXP3+ PD1 PD1 HI CD45RA HELIOS+ Supplementary Figure S2. tSNE analysis of decidual CD4+ T cells. Related to Figure 1. Representative tSNE plots of one decidual CD4+ T cell sample (6-12wk), highlighting CD25, FOXP3, HELIOS, PD1, CD45RA and TIGIT. Three separate CD4+ T cell populations are identified i) CD25 HI FOXP3+HELIOS+, ii) PD1 HI and iii) CD45RA+. TIGIT is co-expressed by C 25 HI and PD1 HI cells but not by CD45RA+ cells. TIGIT is also expressed by a large proportion of the remaining CD4+ T cells, these TIGIT+ cells are clearly identified in Fig 1B bottom panels.