Marilen Benner

THREE TYPES OF TREG CONTROL PLACENTAL INFLAMMATION 77 3 Supplementary Figure S3. IFNγ+, IL-2+ and IL-10+ expression by Treg. Related to Figure 1. Representative histograms of A) IFNγ, B) IL-10 and C) IL-2 expression by CD4+ T cells in blood, decidua 6-12wk and d.parietalis >37wk and in the four T cell subpopulations (CD25 HI , PD1 HI , TIGIT+ and Teff) in decidua 6-12wk. Graphs depict percentage of A) IFNγ+, B) IL-10+ and C) IL-2+ cells within CD4+ T cells (left) and the four T cell subtypes (right). Bars represent median and interquartile range; n= 8-12; *P<0.05, **P<0.01, ***P<0.001. Kruskal-Wallis with Dunn’s post-test comparing the mean ranks to the mean rank of every other column (column A,B,C) within each of the T cell populations. B A CD25 HI PD1 HI TIGIT+ Teff IL-2 CD25 HI PD1 HI TIGIT+ Teff IL-10 CD25 HI PD1 HI TIGIT+ Teff IFNƔ d.parietalis >37wk blood decidua 6-12wk C d.parietalis >37wk blood decidua 6-12wk d.parietalis >37wk blood decidua 6-12wk 0 2 0 4 0 6 0 8 0 1 0 0 % C D 4 + IL - 2 + c e l ls C D 2 5H I P D 1H I TIG IT Te ff 0 2 0 4 0 6 0 8 0 1 0 0 % C D 4 + IL - 2 + c e l ls 0 2 0 4 0 6 0 8 0 1 0 0 % C D 4 + IF N g + c e l ls * * C D 2 5H I P D 1H I TIG IT Te ff 0 5 1 0 1 5 2 0 % C D 4 + IL - 1 0 + c e l ls 0 2 4 6 8 1 0 % C D 4 + IL - 1 0 + c e l ls * * C D 2 5H I P D 1H I TIG IT Te ff 0 2 0 4 0 6 0 8 0 1 0 0 % C D 4 + IF N g + c e l ls * ** * * *** d.parietalis >37wk blood decidua 6-12wk %IL-2+ %IL-2+ %IL-10+ %IL-10+ %IFNγ+ %IFNγ+ CD4 CD25 HI P 1 HI TI IT+ Teff CD4+ CDC4D4 CD25 HI P 1 HI TIGIT+ Teff CD4+ CD4 CD25 HI PD1 HI TIGIT+ Teff CD4+ * * * *** * Supplementary Figure S3. IFN γ +, IL-2+ and IL-10+ expression by Treg. Related to Figure 1. Representative histograms of (A) IFN γ , (B) IL-10 and C) IL-2 expression by CD4+ T cells in blood, decidua 6-12wk and d.parietalis >37wk and in the four T cell subpopulations (CD25 HI , PD1 HI , TIGIT+ and Teff) in decidua 6-12wk. Graphs depict percentage of (A) IFN γ +, B) IL-10+ and (C) IL-2+ cells within CD4+ T cells (left) and the four T cell subtypes (right). Bars represent median and interquartile range; n= 8-12; *P<0.05, **P<0.01, ***P<0.001. Kruskal-Wallis with Dunn’s post-test comparing the mean ranks to the mean rank of every other column (column A,B,C) within each of the T cell populations.