Marilen Benner

THREE TYPES OF TREG CONTROL PLACENTAL INFLAMMATION 79 3 CD45-P.Orange CD8/CD45-A700 CFSE CD4-PerCP CFSE CD4-PerCP CD45-P.Organge CD45/CD8-A700 Teff Teff + Treg Teff Teff + Treg CD4 CD8 Treg R1 CD4 CD8 Treg R1 R1 R1 Teff + TIGIT+ Teff 0.1% 0% 0.1% 0% 0.9% 0% 23% 37% 28% 37% 2.8% 2.8% 27% 33% 4.6% 20% 26% 2.2% 27% 35% 2.4% Teff Teff + CD25 HI Teff +PD1 HI IFNƔ TNFα IL-10 42% 39% 7.3% 29% 27% 1.4% 36% 41% 2.5% IFNɣ TNFɑ IL-10 9.2% 22% 8.3% 6.4% 78% 4.4% 6.0% 5.3% 6.5% CD25 HI PD1 HI TIGIT+ A B C IgG IgG IgG Supplementary Figure S5. Decidual Treg influence cytokine production by CD4+ and CD8+ Teff cells. Related to Figure 3. (A) Gating strategy identifies CD45+CFSE+CD4+ T cells (Blue), CD45+CFSE-CD8+ T cells (Red) and CD45+CFSE-CD8- Treg (green). (B) Representative FACS plots of IFN ɣ , TNF ɑ and IL-10 expression in decidual CD4+ Teff (Blue) and decidual CD8+ Teff (Red) upon addition of CD25 HI , PD1 HI or TIGIT+ Treg compared to CD4+ Teff and CD8+ Teff cultured alone and IgG controls. (C) Representative FACS plots of IFN ɣ , TNF ɑ , IL-10 expression in decidual CD25 HI , PD1 HI or TIGIT+ Treg in the same co-cultures.