Marilen Benner

CHAPTER 3 80 C D 2 5 P D 1 T IG IT T e f f 0 1 0 2 0 3 0 F .C . F O X P 3 m R N A C D 2 5 P D 1 T IG IT T e f f 0 1 0 2 0 3 0 4 0 F .C . C D 2 5 m R N A C D 2 5 P D 1 T IG IT T e f f 0 5 1 0 1 5 2 0 2 5 F .C . P D 1 m R N A C D 2 5 P D 1 T IG IT T e f f 0 1 0 2 0 3 0 4 0 5 0 F .C . T IG IT m R N A blood decidua 6-12wk d.Parietalis >37wk blood ▬ IL18R1 ▬ TIM3 ▬ IRF4 ▬ IFNG ▬ EOS ▬ IL10 ▬ GITR ▬ LRRC32 ▬ CCR8 ▬ CCR5 ▬ ST2 ▬ CD25 ▬ BATF ▬ CCR5 ▬ IFNG ▬ IL10 ▬ CD39 ▬ TIGIT ▬ HLADR ▬ GZMA ▬ TIM3 d.parietalis >37wk decidua 6-12wk decidua 6-12wk blood decidua 6-12wk blood II II CD25 HI PD1 HI TIGIT+ I I II I d.parietalis >37wk d.parietalis >37wk ▬ CCR5 ▬ TNFA ▬ IFNG ▬ IL10 ▬ LRRC32 ▬ GZMA ▬ FOXP3 ▬ CD39 ▬ GITR ▬ PFN ▬ VEGFA ▬ IL18R1 ▬ ICAM1 ▬ EOS B C A Supplementary Figure S6. Gene expression profiles of CD4+ Treg. Related to Figure 4. A) FC mRNA values of CD25, PD1, TIGIT and FOXP3 (n=4). Bars represent median and interquartile range. (B and C) K-means clustering identified two clusters of genes that significantly correlate their mRNA expression pattern in blood, decidua 6-12wk and d.parietalis >37wk. CD25 HI (left panels), PD1 HI (middle panels) and TIGIT+ (right panels) are depicted. Relative Expression Relative Expression Supplementary Figure S6. Gen expression profiles of CD4+ Treg. Related to Figure 4. (A) FC mRNA values of CD25, PD1, TIGIT and FOXP3 (n=4). Bars represent median and interquartile range. (B and C) K-means clustering identified two clusters of genes that significantly correlate their mRNA expression pattern in blood, decidua 6-12wk and d.parietalis >37wk. CD25 HI (left panels), PD1 HI (middle panels) and TIGIT+ (right panels) are depicted.