Marilen Benner

THREE TYPES OF TREG CONTROL PLACENTAL INFLAMMATION 81 3 C D 2 5 H I P D 1 H I T IG IT + T e f f 0 2 0 4 0 6 0 8 0 1 0 0 * * * ** * * * * * * * * * * * * * * * * * * * % C T L A -4 + 0 2 0 4 0 6 0 * * * * * * % G IT R + C D 2 5 H I P D 1 H I T IG IT + T e f f 0 2 0 4 0 6 0 8 0 1 0 0 * * * * * * * * * * * * * * * * * * * * * % G IT R + 0 1 0 2 0 3 0 4 0 * * * * * * * % C T L A -4 + C D 2 5 H I P D 1 H I T IG IT + T e f f 0 2 0 0 0 4 0 0 0 6 0 0 0 8 0 0 0 1 0 0 0 0 * * * * * * * * * * * * * 0 2 0 0 0 4 0 0 0 6 0 0 0 8 0 0 0 * * * * * * Supplementary Figure S7. Expression of CTLA-4, GITR and CD39 by Treg. Related to Figure A) Representative histograms of CTLA-4 (top panels), GITR (middle panels) and CD39 (bott panels) within CD25 HI , PD1 HI , TIGIT+ and Teff cells of blood, decidua 6-12wk, d.basalis >37wk a d.parietalis >37wk. B) Graphs depict percentage of CTLA-4+ (top panels), percentage of GIT (middle panels) and Mean Fluorescence Intensity (MFI) of CD39 (bottom panels) within the to CD4+ cell population and within CD25 HI , PD1 HI , TIGIT+ and Teff cells of blood, decidua 6-12 d.basalis >37wk and d.parietalis >37wk. (n= 8-12) Bars represent median and interquartile ran *P<0.05, **P<0.01 and ***P<0.001. Kruskal-Wallis with Dunn’s post-test comparing all decid fractions (columns B-D) to blood (column A) was performed. B A CTLA4 GITR CD39 decidua 6-12wk d.basalis >37wk d.parietalis >37wk blood CD25 HI PD1 HI TIGIT+ Teff CD25 HI PD1 HI TIGIT+ Teff CD25 HI PD1 HI TIGIT+ Teff d.basalis >37wk d.parietalis >37wk blood decidua 6-12wk CD25 HI HI I f HI HI I I f CD25 HI PD1 HI TIGIT+ Teff %CTLA4+ % ITR+ MFI CD39 %CTLA4+ %GITR+ MFI CD39 CD4+ CD4+ CD4+ Supplementary Figure S7. Expression of CTLA-4, GITR and CD39 by Treg. Related to Figure 4. (A) Representative histograms of CTLA-4 (top panels), GITR (middle panels) and CD39 (bottom panels) within CD25 HI , PD1 HI , TIGIT+ and Teff cells of blood, decidua 6-12wk, d.basalis >37wk and d.parietalis >37wk. (B) Graphs depict percentage of CTLA-4+ (top panels), percentage of GITR+ (middle panels) and Mean Fluorescence Intensity (MFI) of CD39 (bottom panels) within the total CD4+ cell population and within CD25 HI , PD1 HI , TIGIT+ and Teff cells of blood, decidua 6-12wk, d.basalis >37wk and d.parietalis >37wk. (n= 8-12) Bars represent median and interquartile range. *P<0.05, **P<0.01 and ***P<0.001. Kruskal-Walli with Dunn’s post-test comparing all decidual fractions (columns B-D to blood (column A) was perf rmed.