Femke Mathot

7 Motor results of MSC-seeded nerve allografts 119 Autografts Allografts Allografts + undifferentiated MSCs Allografts + differentiated MSCs Axon area • 12 weeks • 16 weeks 27.58 ±2.93% 35.95 ±4.63% 10.14 ±1.89% 21.90 ±3.41% 11.76 ±1.62% 20.07 ±3.18% 15.48 ±2.27% 20.84 ±5.65% Axon count • 12 weeks • 16 weeks 56.87 ±7.47% 50.93 ±7.20% 32.42 ±5.38% 38.94 ±2.67% 39.61 ±5.11% 42.04 ±6.91% 47.39 ±5.55% 43.37 ±13.49% Myelin area • 12 weeks • 16 weeks 57.12 ±4.77% 69.63 ±8.12% 38.43 ±7.18% 57.53 ±6.25% 40.11 ±3.58% 50.69 ±5.72% 46.86 ±4.06% 41.93 ±7.33% Nerve area • 12 weeks • 16 weeks 77.97 ±7.58% 77.37 ±8.55% 63.77 ±5.74% 79.18 ±8.09% 61.58 ±3.53% 66.27 ±7.40% 71.09 ±6.23% 60.74 ±8.77% N-ratio • 12 weeks • 16 weeks 59.06 ±2.48% 72.22 ±2.93% 42.62 ±5.89% 53.57 ±3.04% 46.58 ±3.59% 57.53 ±2.74% 46.58 ±3.59% 51.71 ±5.34% S100 density • 12 weeks • 16 weeks 96.48 ±1.16% 102.37 ±6.94% 97.84 ±1.65% 93.54 ±2.38% 92.85 ±3.17% 94.61 ±3.86% 94.88 ±3.11% 95.92 ±5.90% PGP9.5 density • 12 weeks • 16 weeks 106.95 ±16.30% 92.81 ±5.15% 105.26 ±4.88% 101.89 ±12.22% 101.13 ±9.55% 98.13 ±13.88% 98.62 ±7.97% 76.68 ±6.25% Table 1. Histology and immunofluorescence outcomes obtained at 12 and 16 weeks for all groups. Outcomes are displayed as the mean and the standard error of the mean (SEM). uMSCs = undifferentiated Mesenchymal Stem Cells; dMSCs = differentiated Mesenchymal Stem Cells * = p<0.05, ** = p<0.01, *** = p<0.001. Error bars = Standard error of the mean Figure 6. Examples of obtained images of peroneal nerve sections stained with toluidine blue at 12 weeks of follow-up. Scale bar upper images = 1mm, lower images = 0.5mm.