Femke Mathot

Chapter 7 120 Immunofluorescence S100 and PGP9.5 density outcomes are displayed in table 1 . Examples of obtained immunofluorescence images are displayed in figure 8 . Between group comparisons showed no significant differences in S100 and PGP9.5 density ratio at both time points. Figure 7. N- ratios (R/L) at 12 and 16 weeks. Autografts had a significant higher N-ratio than unseeded allografts and allografts seeded with undifferentiated MSCs at 12 weeks and compared to other groups at 16 weeks. uMSCs = undifferentiated Mesenchymal Stem Cells; dMSCs = differentiated Mesenchymal Stem Cells. * = p<0.05, ** = p<0.01, *** = p<0.001. Error bars = Standard error of the mean Figure 8. An overview of examples of the obtained images of nerve sections stained with S100 (Schwann cells, green) or PGP9.5 (axons, red) (both immunohystochemistry) at 12 weeks. Cell nuclei are DAPI stained and displayed in blue. The displayed images are obtained with 20X magnification, white error bar = 0.2mm.