Tiam Mana Saffari

138 CHAPTER 7 Statistical analysis Testing was performed on both the experimental left side and on the contralateral right side, and results were expressed as a percentage of the values of the left to the right side (L/R ratio in %), to diminish the effect of normal biologic variability between animals. Two-way analysis of variance (two-way ANOVA) for ultrasonography analysis and one-way ANOVA for all other outcomes, corrected by Bonferroni post-hoc testing was used for statistical investigation. Sample size (N=10) was calculated by our statistician based on power analysis to find significant differences in functional recovery between groups. All results were reported as the mean and standard error of the mean (SEM), with level of significance set at α≤0.05. RESULTS Animal weight Differences in sacrifice weight among groups at 12 weeks (P=0.33) and 16 weeks (P=0.23) were not significant. SIEF group All arteries were patent at both sacrifice time points and the flap was actively bleeding during dissection.