Tiam Mana Saffari

202 CHAPTER 9 Immunofluorescence S100 and PGP9.5 density outcomes are displayed in table 1. Examples of obtained immunofluorescence images are displayed in Figure 8. Between group comparisons showed no significant differences in S100 and PGP9.5 density ratio at both time points (Figure 9). Figure 8. An overview of immunofluorescent staining of S100 (Schwann cells, green) and protein gene product 9.5 (PGP9.5, red), respectively. Micrographs showing density in mid-distal graft sections from control nerve samples, autograft, allograft, allografts seeded with undifferentiated mesenchymal stem cells (uMSC) and mesenchymal stem cells differentiated into Schwann cell- like cells (dMSC) at 12 weeks. Cell nuclei are stained with Hoechst (blue) and obtained with 20X magnification. The white scale bar is set at 0.2 mm.