Tiam Mana Saffari

75 OBJECTIVE EVALUATION OF ANGIOGENESIS USING MICRO CT AND PHOTOGRAPHY 4 Figure 1. Schematic drawing of the insertion of the catheter into the rat aorta. Long sutures indicate the grip sutures that have ligated the aorta and vena cava proximally. Short cut suture serves to hold catheter in place while injecting the Microfil® contrast compound into both common iliac arteries of the rat. With permission of the Mayo Foundation, Copyright Mayo Foundation 2019. Outcome measurements Micro CT for calculating the vascular volume After clearing had taken place, the samples were scanned in a SkyScan 1276 micro CT (Bruker Corporation, Billerica, MA, USA) at 40 kV voltage, 200 µA current and 10 µm resolution to calculate vascular volume. Three samples were scanned at a time, taking approximately 26minutes per scan with frame averaging set at three in order to reduce noise. Three-dimensional images of the samples were reconstructed using Hierarchical InstaRecon software (NRecon, 1.7.4.2., InstaRecon, 2.0.4.0. InstaRecon). This software