Dolph Houben

83 Outcomes of vascularized bone allotransplantation 4 Results Pigs were operated at a mean age of 5.8 months with a mean weight of 23.6kg. Over a 20-week period, pigs gained an average of 20.0kg. All animals were able to ambulate immediately with partial weight bearing, with observed full weight bearing without exception by four days (Fig. 2). During the 2-week immunosuppressive period, all pigs were in therapeutic range for Tacrolimus (mean 7.7ng/ml) and Mycophenolate (mean 1.3mcg/ml). Figure 2: normal ambulation and walking 6 weeks after transplantation. Vascular pedicle patency of the allotransplant Doppler ultrasound examination of the transplant vascular pedicle at 2 weeks measured a mean peak systolic velocity of 26.87 cm/s and an end diastolic velocity of 8.68 cm/s (Fig. 3). All vascular pedicles showed a triphasic signal 4 weeks after transplantation. Nine of 14 vascular pedicles showed some non-triphasic blood flow at the 6-week time point, maintained in 3 animals at 6-weeks postoperatively. None of the allogenic vascular pedicles remained patent at week 10. Figure 3: Doppler ultrasound imaging of the vascular pedicle at 4 weeks, showing pulsating blood flow with a mean peak systolic velocity (Mean PS) of 26.87 cm/s and a mean end diastolic velocity of 8.68 cm/s.