M Beerens

WSL ASSESSED ON CLINICAL PHOTOGRAPHY AND BY QLF 2 27 photographs side-by-side. The QLF photographs were assessed for changes in integrated fluorescence loss (IFL) (de Josselin de Jong et al. , 1995). Data collection Subjects whose QLF photographs were used fulfilled the following criteria: 1. treated with orthodontic fixed appliances in both arches with debond between January 2008 and August 2009, 2. healthy and between the age of 12–19 at debond, and 3. have developed two or more WSL without open cavitation on previously bracketed buccal surfaces. All subjects who completed the caries clinical trial with QLF photographs available from T1 and T2 were eligible for enrolment in the current study. Subjects were enrolled after having signed informed consent, which, in case of minors was co-signed by parents/guardians. The clinical photographs of these subjects at the same time points were retrieved from the patient database. Only complete datasets were considered. Furthermore, clinical and QLF photographs should be available from T1 and T2. Subjects were enrolled after signed informed consent, which was in the case of minors co-signed by parents/ guardians. Clinical Oral Photograph galleries Clinical oral photographs were captured using a Nikon digital camera D3000 camera body with CCD (charge coupled device), DCS (digital still camera) from Kodak (Odijk, The Netherlands) with an image size of 1012 x 1524 pixels or 4.5 Mb. The camera had a 2.8/105 mm macro lens (that combined with the CCD surface size, resulted in images comparable to those made with a 160- mm lens). The camera was equipped with a Nikon SB 29 s macro speed light flasher. Photographs were stored on a computer. At each time point a set of front teeth in maximal occlusion, front teeth in open position; left and right lateral views in occlusion were captured. Frontal pictures were captured under an angle of 0° in maximal occlusion and in opened position approximately 3 mm from occlusion. Left and right lateral pictures were captured under an angle of 45° (Figure 1). Frontal pictures were captured using a rounded cheek retractor (DB orthodontics, Silsden, UK, Set large. Double end large DB04-0175). Oval shape

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