M Beerens

EFFECTS OF MI PASTE PLUS ® : A 3-MONTHS FOLLOW-UP 4 59 animal, and human in situ experimental studies (Reynolds, 1987; Shen et al. , 2001; Andersson et al. , 2007; Oshiro et al. , 2007; Cochrane et al. , 2008; Rahiotis et al. , 2008; Walker et al. , 2009). Two clinical trials on the remineralisation of post-orthodontic WSL by CPP-ACP cream show contrasting findings (Bailey et al. , 2009; Bröchner et al. , 2011). The use of CPP-ACPF has thus far only been reported in in vitro experiments (Zhao and Cai, 2001; Cross et al. , 2004; Cochrane et al. , 2008). Therefore, the aim of this study was to investigate the effects of commercially available CPP-ACPF paste (MI Paste Plus®) in vivo on dental plaque and on the remineralisation of enamel WSL as assessed by QLF after the removal of fixed orthodontic appliances, in a randomized controlled clinical trial during a 3-month time period. The null hypothesis was that the use of CPP-ACPF paste (MI Paste Plus®) in vivo , in addition to normal oral hygiene, does not have an effect on (i) the remineralisation of subsurface lesions and/or (ii) plaque composition (expressed as the percentage of aciduric bacteria, S. mutans, and Lactobacillus spp.) in orthodontic patients with WSL after the removal of fixed orthodontic appliances. MATERIAL AND METHODS The study was performed as a prospective, double blinded, randomized placebo controlled clinical trial. Aiming to to determine the effect of a CPP- ACPF paste vs. a control paste on plaque composition and remineralisation of enamel WSL in subjects with multiple WSL at the end of treatment with a fixed appliance. The trial was conducted during the first 3 months following appliance removal. The medical ethical committee of the University Medical Centre of the Free University Amsterdam, the Netherlands, approved this study protocol (MEC 07/213). Subjects Orthodontic patients treated with full fixed appliances in both arches, which were scheduled for debonding, were invited to participate in this study and were debonded and screened for WSL between January 2008 and August 2009 at the Department of Orthodontics, Academic Centre of Dentistry Amsterdam, the Netherlands.