M Beerens

EFFECTS OF MI PASTE PLUS ® : A 12-MONTHS FOLLOW-UP 5 91 A repeated-measures ANOVA with a Greenhouse–Geisser correction showed a significant improvement in fluorescence loss (∆ F ) over time [ F (3.014, 31.346) = 17.155, P < 0.001; for MI Paste Plus ® group, F (2.659, 19.705) = 11.533, P < 0.001; and for the control group F (3.097, 14.120) = 6.757, P < 0.001], but no significant differences between the two groups. Multiple comparisons with baseline using Bonferroni correction showed significant differences of the fluorescence loss (∆ F ) in the MI Paste Plus ® group from baseline to T4 ( P = 0.021) and T5 (P < 0.001) and from T2 to T5 (P = 0.002) and the control group from baseline to T5 (P = 0.002). Repeated-measures ANOVA showed no significant changes in the IFL over time or between the groups. In both groups, a trend of improvement in IFL was seen. Microbial composition of plaque Microbial composition of plaque (secondary outcome) described by CFUs (counts/sample), proportions of aciduric bacteria [% bacteria count/total count], S. mutans [% bacteria count/total count], Lactobacillus spp. [% bacteria count/ total count], and the fungus C. albicans [% fungi count/total count] is given in Table 3. At T0 (baseline for microbial composition), no significant differences between groups were found ( P > 0.05). Five participants in the control group were excluded from the analysis due to missing data at T5 (1 year). Of these five participants no plaque was present on the surface in four cases. In one case, the sample was lost. Total CFUs did not change significantly over time and were not different between the groups (repeated-measures ANOVA, P > 0.05). Repeated- measures ANOVA with a Greenhouse-Geisser correction showed a significant reduction in percentage of aciduric bacteria over time in the Mi Paste Plus ® but not in the control group (MI Paste Plus ® group F (2.700, 2829.398) = 2.916, P < 0.047; control group F (2.763, 3149.550) = 1.853, P > 0.05). No significant differences between the groups ( P > 0.05) were found. Multiple comparisons with baseline using Bonferroni correction showed significant differences in the reduction in percentage of aciduric bacteria from baseline to T5 in the MI Paste Plus ® group (Table 3). A similar trend was seen in the control group, although not significant over time. Repeated-measures ANOVA with Greenhouse–Geisser correction showed no significant changes in the the reduction in percentage of S. mutans over time