M Beerens

92 CHAPTER 5 for the MI Paste Plus ® group, but significant changes in time were found for the control group (MI Paste Plus ® group F (3.048, 229.581)= 1.728, P > 0.05; control group F (2.347, 625.465)= 3.236, P = 0.039). No significant differences between the groups ( P > 0.05) were found. The reduction in percentage of Lactobacillus spp. and C. albicans did not change significantly over time or between the groups (repeated-measures ANOVA with Bonferroni correction, P > 0.05). TABLE 3. Microbial composition, determined by total bacterial counts, the proportions of aciduric bacteria, Streptococcus mutans spp., Lactobacillus spp. and Candida Albicans, at five different time points in the MI Paste Plus ® group and the control group. (T0= before debonding, T2= 6 weeks, T3= 3 months, T4= 6 months, and T5= 12 months after debonding) MI Paste Plus ® , N = 25 T0 T2 T3 T4 T5 Colony-forming units (CFUs) (10 7 ) counts/sample 5.4 ± 6.3 4.3 ± 4.8 4.3 ± 4.4 4.2 ± 5.2 5.5 ± 4.3 Aciduric bacteria (% bacteria count/total count) 53.2 ± 33.5 47.2 ± 31.7 46.4 ± 27.8 32.9 ± 27.1 29.3 ± 19.2 * Streptococcus mutans (% bacteria count/total count) 9.8 ± 14.1 3.9 ± 7.4 8.6 ± 10.5 4.7 ± 9.6 8.6 ± 13.6 Lactobacillus spp. (% bacteria count/total count) 0.2 ± 0.5 0.0 ± 0.1 0.1 ± 0.2 0.0 ± 0.1 0.1 ± 0.2 Candida Albicans (% fungi count/total count) 1.0 ± 2.2 0.5 ± 1.9 0.7 ± 1.8 0.2 ± 0.9 1.0 ± 4.4 Control, N = 26 T0 T2 T3 T4 T5 Colony-forming units (CFUs) (10 7 ) counts/sample 3.4 ± 3.2 3.6 ± 3.6 5.1 ± 4.6 8.8 ± 2.7 6.0 ± 9.5 Aciduric bacteria (% bacteria count/total count) 49.2 ± 49.4 48.0 ± 38.4 32.2 ± 25.3 34.4 ± 27.6 31.3 ± 21.2 Streptococcus mutans (% bacteria count/total count) 12.2 ± 19.5 4.3 ± 7.9 4.2 ± 9.2 10.8 ± 16.5 5.9 ± 9.0 Lactobacillus spp. (% bacteria count/total count) 0.1 ± 0.2 0.4 ± 1.8 0.3 ± 0.9 0.0 ± 0.0 0.0 ± 0.0 Candida Albicans (% fungi count/total count) 5.7 ± 15.2 11.5 ± 57.2 2.0 ± 7.6 0.9 ± 2.0 0.2 ± 1.0 Bacterial counts are expressed as a percentage of the total counts per sample obtained at each time point. Data are given as mean ± SD. *Data significantly different from baseline.