M Beerens

EFFECTS OF MI PASTE PLUS ® : A 12-MONTHS FOLLOW-UP 5 93 Acidogenicity of plaque Acidogenicity of plaque (secondary outcome) was determined as the amount [µmol acid/mg protein] of formate, succinate, acetate, lactate, propionate, butyrate, and phosphate in resting plaque and after 10 minutes of sucrose rinse (Supplementary Figure S). No significant differences between the groups were found for any acid at baseline. Phosphate was significantly lower in the MI Paste Plus ® group in comparison with the control group at baseline (MI Paste Plus ® = 0.40, SD = 0.21; control group = 0.57, SD = 0.34, P = 0.04). No significant differences in acid and phosphate composition of resting plaque or after sucrose pulse were seen in time or between the groups (repeated- measures ANOVA, P > 0.05). Lesion changes assessed by clinical oral photographs Changes in enamel lesions between T1 and T5 (secondary outcome) assessed on the clinical photographs (Figure 1) are shown in Table 4. Three participants were excluded, one in the MI Paste Plus ® and two in the control group. These participants had an incomplete photograph gallery at T5. No significant differences were found between the groups at baseline (Mann- Whitney U ; mean U = 79 980, z = –4,54, P = 0.001), showing less visible lesions in the MI Paste Plus ® group than in the control group. Most of the surfaces were scored 0 for both the MI Paste Plus ® and the control group at T1. There was no significant difference between the groups over time. Lesions that scored 2 essentially did not changed over time. One lesion was given an ICDAS score of 3 on the photograph gallery. This lesion was assessed as ICDAS score 2 clinically. The lesion in the control group that scored 3 at T1 and 0 at T5 has been restored with a filling and appeared undetectable.The lesions that scored 0 at T1 and 2 at T5 are presumably lesions that appeared after gingiva reduction.