15283-B-Blokker

130 Chapter 7 TABLES Table 1. GAPDH primer sequences and base pair lengths used for RT-qPCR analyses Primer Primer sequence (5' --> 3') Amplicon size PCR efficiency Base Pairs POWER (10(-1/slope))* GAPDH common fwd CCA CAT CGC TCA GAC ACC AT GAPDH rev ACC AGG CGC CCA ATA CG 71 1,94 GAPDH rev GTA AAC CAT GTA GTT GAG GTC 153 1,93 GAPDH rev TTG ACG GTG CCA TGG AAT TT 200 1,86 GAPDH rev ACT TGA TTT TGG AGG GAT CT 277 1,81 GAPDH rev AAG ACG CCA GTG GAC TCC A 323 1,82 GAPDH rev ACG ATA CCA AAG TTG TCA TG 530 1,71 * based on a 4 fold dilution series of pooled fresh frozen tissue derived cDNA samples Table 2. Number of tissue samples per sample type per tissue type Tissue type Sample type Total Fresh Frozen MIA CA Heart 25* 25* 24 74 Kidney 24 24 24 72 Liver 24 24 24 72 Total 73 73 72 218 * extra samples collected