15283-B-Blokker

132 Chapter 7 Table 5. Morphologic tissue quality assessment per sample type Sample type Fresh frozen n = 73 MIA n = 73 CA n = 72 Representative yes 88% 88% 100% no 5% 0% 0% No H&E-slide* 7% 12% 0% n = 68 n = 64 n = 72 Autolysis severe 3% 11% 21% moderate 16% 50% 44% no 81% 39% 35% Necrosis yes 3% 3% 6% no 93% 94% 86% n/s** 4% 3% 8% * No H&E-slide, all tissue used for RNA isolation ** n/s = no scoring possible Table 6. P-values comparing sample types per GAPDH size assay Sample type P -value 71 bp 153 bp 200 bp 277 bp 323 bp 530 bp Fresh frozen vs. MIA <0 .001 <0.001 <0.001 <0.001 <0.001 <0.001 Fresh frozen vs. CA <0 .001 <0.001 <0.001 <0.001 <0.001 <0.001 MIA vs. CA 0.009 0.003 0.005 0.009 0.003 0.016 Kruskal Wallis, significance level 0,00833