15283-B-Blokker

134 Chapter 7 Figure 2. GAPDH size assay in fresh frozen, MIA and CA samples. Legend: The median Cq values (X-axis) of RNA derived from fresh frozen, MIA and CA samples obtained by RT-qPCR with various PCR product lengths (Y-axis) are shown. The fresh frozen samples always show lower Cq values, whereas the MIA samples show intermediate and the CA samples show high Cq values. This indicates that RNA in MIA samples is less degraded than RNA in CA samples. The parallel increasing Cq values between GAPDH323 and GAPDH530 indicate suboptimal RT-qPCR performance, rather than decreased GAPDH expression levels.