15289-s-bos

8 131 | Novel protein biomarker predictors of CAD in FH Table 8.4 | Tests comparing ROC AUC Predictor of CVD AUC 95% CI for AUC s.e. LCL UCL LRG1 (as measured) 0.911 0.838 0.984 0.037 LRG1 (zero vs. more than zero) 0.911 0.839 0.983 0.037 ITIH3 (as measured) 0.784 0.693 0.875 0.046 ITIH3 (zero vs. more than zero) 0.783 0.691 0.874 0.047 C4B (as measured) 0.918 0.857 0.979 0.031 C4B (zero vs. more than zero) 0.898 0.823 0.974 0.039 C1QB (as measured) 0.905 0.823 0.986 0.041 C1QB (zero vs. more than zero) 0.911 0.839 0.983 0.037 CD14 (as measured) 0.654 0.580 0.727 0.037 CD14 (zero vs. more than zero) 0.654 0.580 0.727 0.037 HRG (as measured) 0.922 0.862 0.983 0.031 HRG (zero vs. more than zero) 0.911 0.839 0.983 0.037 Total peptides detected 0.912 0.840 0.983 0.037 Any peptides detected 0.911 0.839 0.983 0.037 Predictive accuracy of proteins with disease severity using area under the ROC (receiver operating curve), to compare peptides with each other. AUC: Area under curve, LCL: Lower confidence level, UCL: Upper confidence level, s.e.: Standard error, LRG1: Leucine-rich alpha-2-glycoprotein, ITIH3: Inter-alpha-trypsin inhibitor heavy chain H3, C4B: Complement C4-B C1QB: Complement C1q subcomponent subunit B, CD14: Monocyte differentiation antigen, HRG: Histidine- rich glycoprotein Overall ROC analysis revealed LRG1, C4B and C1QB were excellent indicators of CAD in patients with FH (AUROC > 0.9), with ITIH3 only a relatively good indicator. In addition, the total number of proteins detected and whether any of themwere detected was also excellent. For indication of either coronary atherosclerosis or CAD; LRG1, C4B, C1QB and HRG were relatively good (Figure 3). Although C1QB is a possible indicator, none of the protein biomarkers were considered to be good for indicating coronary atherosclerosis alone. When the AUC is adjusted for the use of RR-lowering drugs, bootstrapped estimates of the AUC showed that CD14 and ITIF3 are not accurate indicators of CAD when adjusted for the presence of RR-lowering drugs, but HRG (AUC=0.920, 0.846-0.974) and C4B (0.916, 0.837-0.961) are excellent indicators of CAD and are little influenced by RR-lowering drugs.