Mark Wefers Bettink

Chapter 9 170 general anesthesia with sevoflurane/midazolam, rocuronium and fentanyl. MitoPO 2 measurements started before surgery and continued until after surgery. After completion of the measurement the primed skin was shielded against light with an aluminum plaster for 48 hours. This is based on the pharmacological characteristics of ALA. The mean half- life fluorescence clearance of PpIX is 30 ± 10 hours. Results Informedconsentwasobtained in11CDHand4EApatients. Intraoperativemeasurements were performed in all 15 included neonates. Neonates had a median gestational age of 38 weeks (IQR 37,7 – 40,2), a median birth weight of 3,000 grams (IQR 2,400-3,340) and a median age at surgery of 2 days (IQR 2-5.5). Median duration of the surgical procedure was 106 minutes (IQR 95-116) and two patients received surgical repair of CDH on ECMO in the pediatric intensive care unit (Table 1). Median skin priming time with ALA was 7h45m (IQR 6h50m – 12h0m). Twelve out of 15 measurements were successful with a median duration of the MitoPO 2 measurement of 116 minutes (IQR 98-133) (Table 1). The first measurement failed due to the radiant warmer (infra-red light), the second due to pink chlorohexidine-alcohol disinfectants and the third due to purple skin marker on the primed skin. Table 1. Patient demographics n=15 Median (IQR) Male gender, n (%) 8 (53%) Gestational age, wk 38.1 (37.7 – 40.2) Birth weight, grams 3000 (2400 – 3340) Age at surgery, days 2 (2 – 5.5) Duration of surgery, min 106 (95-116) Priming time skin, min 465 (413 – 720) Duration MitoPO 2 measurement 116 (98 – 133) Surgical approach Thoracoscopy, n (%) 5 (33%) Thoracotomy, n (%) 2 (13%) Laparotomy, n (%) 8 (53%) Surgery during ECMO, n (%) 2 (13%)