Mark Wefers Bettink

Verification of calibration in man and comparison with vascular occlusion tests in healthy volunteers with COMET 4 73 Application of cyanide cream on the skin in the first session led to low signal quality and, except for one case, mitoPO 2 readings well below the corresponding PaO 2 values (Figure 6a). This COMET behavior was analyzed in cooperation with the manufacturer and appeared to be caused by the detector gating. Due to the specific timing of this gating, the very short delayed fluorescence lifetimes resulting from the high intracellular PO 2 were omitted from the signal analysis, leading to an erroneously low steady mitoPO 2 around 66 mmHg. Figure 6: Comparison between COMET mitoPO 2 after cyanide cream had been applied to block mitochondrial respiration and arterial blood gas PaO 2 taken from a radialis. A) Data from session 1, even though the delayed fluorescence was insufficient a mitoPO 2 was displayed around 65 mmHg. One subject with a strong signal the short lifetime was measurable, seen as black dot 126 mmHg. B) Data from session 2 with adjusted software. No significant difference was seen in session 2 between mitoPO 2 and PaO 2 . To enable accurate detection of high mitoPO 2 values the timing of the gating was adjusted by a temporary adaptation in the firmware. During session 2 data was collected with this adjusted software. Now, the median [IQR] mitoPO 2 was 94.1 mmHg [87.2 – 110.9] and